java.lang.RuntimeException: Error running OptiFine patcher at E:\minecraft.minecraft\versions\1.16.5-Fabric 0.14.25-OptiFine_G8\mods\OptiFine_1.16.5_HD_U_G8.jar on E:\minecraft.minecraft\versions\1.16.5-Fabric 0.14.25-OptiFine_G8\1.16.5-Fabric 0.14.25-OptiFine_G8.jar
at me.modmuss50.optifabric.mod.OptifineSetup.runInstaller(OptifineSetup.java:214)
at me.modmuss50.optifabric.mod.OptifineSetup.getRuntime(OptifineSetup.java:108)
at me.modmuss50.optifabric.mod.OptifabricSetup.run(OptifabricSetup.java:46)
at com.chocohead.mm.Plugin$$Lambda$46/1618984457.accept(Unknown Source)
at java.util.ArrayList.forEach(ArrayList.java:1249)
at com.chocohead.mm.Plugin.getMixins(Plugin.java:340)
at org.spongepowered.asm.mixin.transformer.PluginHandle.getMixins(PluginHandle.java:128)
at org.spongepowered.asm.mixin.transformer.MixinConfig.postInitialise(MixinConfig.java:796)
at org.spongepowered.asm.mixin.transformer.MixinProcessor.prepareConfigs(MixinProcessor.java:568)
at org.spongepowered.asm.mixin.transformer.MixinProcessor.select(MixinProcessor.java:462)
at org.spongepowered.asm.mixin.transformer.MixinProcessor.checkSelect(MixinProcessor.java:438)
at org.spongepowered.asm.mixin.transformer.MixinProcessor.applyMixins(MixinProcessor.java:290)
at org.spongepowered.asm.mixin.transformer.MixinTransformer.transformClass(MixinTransformer.java:234)
at org.spongepowered.asm.mixin.transformer.MixinTransformer.transformClassBytes(MixinTransformer.java:202)
at net.fabricmc.loader.impl.launch.knot.KnotClassDelegate.getPostMixinClassByteArray(KnotClassDelegate.java:422)
at net.fabricmc.loader.impl.launch.knot.KnotClassDelegate.tryLoadClass(KnotClassDelegate.java:323)
at net.fabricmc.loader.impl.launch.knot.KnotClassDelegate.loadClass(KnotClassDelegate.java:218)
at net.fabricmc.loader.impl.launch.knot.KnotClassLoader.loadClass(KnotClassLoader.java:119)
at java.lang.ClassLoader.loadClass(ClassLoader.java:357)
at java.lang.Class.forName0(Native Method)
at java.lang.Class.forName(Class.java:348)
at net.fabricmc.loader.impl.util.DefaultLanguageAdapter.create(DefaultLanguageAdapter.java:50)
at net.fabricmc.loader.impl.entrypoint.EntrypointStorage$NewEntry.getOrCreate(EntrypointStorage.java:117)
at net.fabricmc.loader.impl.entrypoint.EntrypointContainerImpl.getEntrypoint(EntrypointContainerImpl.java:53)
at net.fabricmc.loader.impl.FabricLoaderImpl.invokeEntrypoints(FabricLoaderImpl.java:383)
at net.fabricmc.loader.impl.launch.knot.Knot.init(Knot.java:160)
at net.fabricmc.loader.impl.launch.knot.Knot.launch(Knot.java:68)
at net.fabricmc.loader.impl.launch.knot.KnotClient.main(KnotClient.java:23)
at sun.reflect.NativeMethodAccessorImpl.invoke0(Native Method)
at sun.reflect.NativeMethodAccessorImpl.invoke(NativeMethodAccessorImpl.java:62)
at sun.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43)
at java.lang.reflect.Method.invoke(Method.java:497)
at oolloo.jlw.Wrapper.invokeMain(Wrapper.java:71)
at oolloo.jlw.Wrapper.main(Wrapper.java:51)
Caused by: java.lang.ClassNotFoundException: optifine.Patcher
at java.net.URLClassLoader$1.run(URLClassLoader.java:370)
at java.net.URLClassLoader$1.run(URLClassLoader.java:362)
at java.security.AccessController.doPrivileged(Native Method)
at java.net.URLClassLoader.findClass(URLClassLoader.java:361)
at java.lang.ClassLoader.loadClass(ClassLoader.java:424)
at java.lang.ClassLoader.loadClass(ClassLoader.java:357)
at me.modmuss50.optifabric.mod.OptifineSetup.runInstaller(OptifineSetup.java:210)
... 33 more
Caused by: java.util.zip.ZipException: invalid LOC header (bad signature)
at java.util.zip.ZipFile.read(Native Method)
at java.util.zip.ZipFile.access$1400(ZipFile.java:61)
at java.util.zip.ZipFile$ZipFileInputStream.read(ZipFile.java:717)
at java.util.zip.ZipFile$ZipFileInflaterInputStream.fill(ZipFile.java:420)
at java.util.zip.InflaterInputStream.read(InflaterInputStream.java:158)
at sun.misc.Resource.getBytes(Resource.java:124)
at java.net.URLClassLoader.defineClass(URLClassLoader.java:462)
at java.net.URLClassLoader.access$100(URLClassLoader.java:73)
at java.net.URLClassLoader$1.run(URLClassLoader.java:368)
... 39 more

I suspect your OptiFine jar file is corrupt. Download it again.

Why are you using a custom jar file?

I don't know what you mean

E:\minecraft.minecraft\versions\1.16.5-Fabric 0.14.25-OptiFine_G8\1.16.5-Fabric 0.14.25-OptiFine_G8.jar

^^ That is a custom jar and not the MC vanilla 1.16.5 jar file.