After i started MC, tewakeroo suddenly didnt want to work anymore

I've had this issue many times before.
I don't know if it is still an issue but maybe you should try updating all of your mods.
That has worked for me in the past.

This worked for me! Thanks!

For me, Windows + G and then recording a video causes all the Tweakaroo sort cuts to stop working. Restarting the game by running a new instance of the launcher seems to fix it again

@BugReporterNova I assume the windows key (or maybe G) gets stuck then. Try pressing both of those again while in-game. You can also use the keybindDebugging option from malilib (A + C or via Mod Menu) to see what keys it currently sees as pressed.

You didn't provide any information whatsoever, what am I supposed to know and respond based on that?

How does it not work? Do none of the hotkeys respond? Is the mod still loaded? If you have Mod Menu, do you see Tweakeroo in the mod list? Is Fabric still working/loaded (shown in the bottom left of the main menu and in the top left of the F3 screen)?

None off the hotkeys respond, the options menue cant be open. Fabric is still working. Loaded mods are: optifine, litematica, minihund, malilib, tweakeroo and world edit

One possibility for no hotkeys working is that you have some key "stuck". This can happen for example if you Alt + Tab out of the game, as the game may then not see the key release event for Alt for example. So as far as malilib sees, the key is then left being pressed even if it's physically not. Pressing the key again will fix this issue.

There is also a Debug -> keybindDebugging option in malilib configs (A + C) to see if you have keys stuck. But to be able to open that config menu when you have a key stuck, you would either need to set that menu hotkey to Allow Extra Keys beforehand, or use the Mod Menu mod and the config menu button in there to open the config menu.

I would recommend the Mod Menu mod anyway, as it gives you a fall-back way to open the mod config menus via the mod list. You can get it from here: https://www.curseforge.com/minecraft/mc-mods/modmenu/files

The Debug option in malilib shows that everything is working fine :/

No keys are getting stuck down? Then did you change some of the Tweakeroo hotkeys? Is Tweakeroo loaded according to Mod Menu? (Or alternatively do you see a message about Fabric loading Tweakeroo in the console/log file?)

Tweakeroo is loaded according to ModMenu, as i know i didnt change a single hotkey of Tweakeroo. It suddenly stopped working from one to the other day

I've had this issue many times before.
I don't know if it is still an issue but maybe you should try updating all of your mods.
That has worked for me in the past.